1. The only rapid FISH probes that have obtained both NMPA and CE certifications;
2. The accompanying FISH pre-treatment reagents (sample extraction agents) are environmentally friendly degreasing agents and highly effective permeabilizing agents, which improve the first-pass detection rate of FISH and reduce probe reagent costs;
3. High specificity, sensitivity, and accuracy: the probes are based on non-repetitive sequence technology, which removes repetitive sequences present in the genome, thereby reducing non-specific hybridization with the target specimen, lowering fluorescent background, and enhancing the specificity, sensitivity, and accuracy of probe detection;
4. Simplicity and speed: the total processing time required by this kit is 2 hours, compared to 16 hours required by commercially available fluorescence in situ hybridization (FISH) probe kits, significantly reducing hybridization time; the workflow is straightforward, and reports can be issued on the same day;
5. Excellent anti-contamination performance: after detection, the probes can be stored at -20°C in a freezer for more than six months.

Rapid hybridization is a proprietary technology independently developed by Kanlume, for which a national invention patent has been granted. The specific implementation method is a trade secret.However, the basic principles of rapid hybridization can be shared as follows:
1.The probe itself employs a repeat-depletion technique, containing no repetitive sequences. As a result, the probe is small and has few complex secondary structures, allowing it to cross the nuclear membrane and achieve hybridization binding faster than traditional probes.
2.The pre-treatment reagents are specially developed for the rapid probes, ensuring hybridization efficiency and reliable experimental outcomes.

The genomic DNA of eukaryotic organisms consists of unique DNA sequences ( non-repetitive sequences) and sequences that are present in multiple copies (repetitive sequences). The traditional FISH probe design method involves fluorescently labeling a large DNA fragment (containing both non-repetitive and repetitive sequences) that includes the target gene sequence, and then using Human Cot-1 DNA to block the repetitive sequences, thereby enabling detection of the target gene.HealthCare Biotechnology’s design, in contrast, removes the repetitive sequences from the genomic DNA sequence and then fluorescently labels the remaining non-repetitive sequences to generate the probe. This method is known as non-repetitive sequence technology.

Kanlume’s probes are designed using non-repetitive sequence technology and contain no repetitive sequences. As a result, the fluorescence background is weak, background noise is reduced, hybridization signals are clearer, and result interpretation is more accurate.

1.Rapid hybridization can be completed in just 2 hours. Samples received in the morning can be read before the end of the afternoon shift, thereby improving testing efficiency.
2. Kanlume’s rapid FISH probes allow flexible hybridization durations, ranging from 24 hours to overnight, with consistent results. If samples arrive in the afternoon, overnight hybridization enables results to be obtained the next day. This feature allows the laboratory department to flexibly plan experimental schedules using Kanlume’s probes.
3.Because FISH experiments have high demands on sample quality, poor-quality samples require retesting. The rapid FISH probes enable rapid completion of retesting when needed, minimizing the waiting time for results by clinical departments and patients.

Kanlume’s pretreatment reagents are specifically developed as supporting reagents for rapid FISH probes. Only when section samples are treated with these reagents and subsequently tested with Kanlume probes can rapid hybridization be achieved. Furthermore, to ensure hybridization speed and performance, it is essential to follow the accompanying pretreatment procedure provided by Kanlume and use the matched pretreatment reagents. In theory, good results can also be obtained without using Kanlume’s pretreatment reagents; however, rapid hybridization effects cannot be guaranteed.

Kanlume’s fully automated FISH pretreatment system completes all the work from the arrival of tissue section samples at the laboratory to the point just before probe dispensing, all automatically. This device does not have hybridization capability; the sealed slides need to be placed into a hybridization instrument for the hybridization process.

During sample pretreatment, the reagents are enclosed. Except for alcohol and deionized water,Kanlume’s special extractant must be used. For FISH hybridization after pretreatment, commonly available FISH probes on the market can be used. However, since this device and the sample extractant are developed in conjunction with Kanlume’s own rapid FISH probes, it is recommended to use Kanlume's probes to achieve optimal detection performance.